The new genotypes, which increased the allelic diversity and heterozygosity in Gombe by 61% and 8%, respectively, were validated by replicate amplification and pedigree analyses. Applying this method to the entire Gombe population, we confirmed previously reported genotypes, but also identified 31 new alleles that had been missed due to sequence differences and size homoplasy. Using data from eight microsatellite loci as a reference, we designed a bioinformatics platform that converts raw MiSeq reads into locus‐specific files and automatically calls alleles after filtering stutter sequences and other PCR artifacts. Here we developed a MiSeq‐based approach and tested its performance using previously genotyped fecal samples from long‐term studied chimpanzees in Gombe National Park, Tanzania. However, this technique is labor intensive, difficult to compare across platforms, and notoriously imprecise. Until recently, capillary electrophoresis has been the method of choice to determine the length of polymorphic STR loci. The support agent will provide you with a Code for the Code box and further instructions.Short tandem repeats (STRs), also known as microsatellites, are commonly used to noninvasively genotype wild‐living endangered species, including African apes. Inform the support agent that you just completed a VERUS Re-Image and are at. It is located on a white barcode sticker adhered to the back of the unit.Ĭontact Snap-on Diagnostics Technical Support at 80. In the “Your Name:” box enter the VERUS serial number. Click on the “Exit” button.Įstablish a connection between the VERUS and the internet. On the VERUS Server popup select “Disable launch at startup” check box. Press “OK” to reboot.Īfter the VERUS boots press “OK” on the CommPort error pop up box. This Process will take approximately 10-15 min. Press “OK” on the System Recovery popup box. Continue to hold the Left “Alt” button down until the “Loading RAMDISK Image” screen is displayed. Press and hold the LEFT “Alt” button down and then press the VERUS’ power button once. The VERUS will not resume normal operation until it has been successfully connected to the internet and Snap-on Diagnostics Technical Support has been allowed to remotely access the unit. This process requires access to a USB Keyboard and an Internet connection to the VERUS. All 3rd party software will be uninstalled and/or deleted.ĭo not perform this process unless you have been instructed to do so by a Snap-on Representative. All saved user files and documents will be deleted. WARNING: This procedure will restore the unit to its factory settings.
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January 2023
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